AUTOMATED AND MANUAL SEMEN ANALYSIS: THE COMPARATIVE CHARACTERISTICS

Currently, spermogram parameters are routine criteria for assessing male fertility. There two methods of semen analysis: traditional manual and automated. The concentration spermatozoa various motility categories is important in predicting physiological conception, as well choosing fertilization assisted reproductive technology programs. introduction automatic analyzers has shown that their use can be considered an alternative to the analysis method, which contribute laboratory standardization. Initially, these devices demonstrated difficulty accurately indicating sperm due presence aggregation large amounts cellular debris. In present study, main focus was on by automated methods. A total 50 samples were analyzed from patients participating Manual performed a Makler's chamber according standard method 10 small squares. each spermatozoon classified into categories. Automated carried out using CASA analyzer computer system (MICROPTIC, Spain), uses principle microscopic imaging processing detect motile immobile through fast consistent images. Statistical independent variables chosen evaluate obtained data. As part determining confidence intervals, statistically significant difference found between or when evaluating with highest speed 0,025 mm/sec rectilinear translational movement, lower speed, either aging broken morphology. Most likely, this objective visual assessment such data suggest higher degree objectivity mobile biological objects, particular germ cells.